Links for Flow Cytometry
Training on the Accuri C6,
LSRFortessa and FACSCanto
Life Technologies Fluorescence and Flow Cytometry Tutorials
BD Biosciences Introduction to Flow Cytometry
Multicolor Flow Cytometry: The Brilliant Violet Family of Fluorophores (Kelly Lundsten; BioLegend)
Compensation, an informal perspective (Mario Roederer; modified May 24, 2000)
ABCore Instruments
Lasers
and Detectors for Aria, Canto, Fortessa and Accuri C6 pdf
BD LSR Fortessa (brochure pdf) or LSR Fortessa (general information)
FACS Aria II (brochure pdf) or FACSAria II (general information)
Accuri
C6 (brochure pdf) and Accuri
C6 (general information)
FACS Canto (brochure pdf)
Additional Flow Tutorials
Introduction to Flow Cytometry I (J. Paul Robinson PhD; PUCL)
Introduction to Flow Cytometry II (J. Paul Robinson PhD; PUCL)
Software Tutorials and User Guides
FACSDiva v6 Software Overview Course (BD Biosciences)
Accuri C6 Software User Guide.pdf
BD CSampler Software User Guide.pdf
WinMDI 2.8 download and tutorial
Books
Practical Flow Cytometry (Howard Shapiro); free download with registration
Flow Cytometry - A Basic Introduction (Michael G. Ormerod)
Instrument User Guides
Accuri C6 Flow Cytometer Instrument Manual.pdf
Organizations and Flow User Group
PUCL Purdue University Cytometry Laboratories. Lots of flow information and the Purdue Cytometry Discussion List (new and archived threads covering many topics; threads are from novice users as well as experienced users)
International Society for Advancement of Cytometry (
Southeast Flow Cytometry Interest Group
Compensation and Controls
Compensation, an informal perspective (Mario Roederer; modified May 24, 2000)
Fluorescence Compensation.pdf (Jennifer Wilshire, PhD; FlowJo) Besides compensation, this article goes over setting up FMOÕs
Compensation: The gruesome details (Mario Roederer; modified May 24, 2000)
Compensation: It's not just for pretty pictures.pdf (Mario Roederer)
General Information
BD Biosciences Flow Cytometry – application notes, webinars, protocols
Life Technologies Spectral Viewer
BD Biosciences Multicolor Panel Designer – see first link for examples of Mouse and Human panels
FluoroFinder Panel Designer – resource to streamline panel design tailored for cytometers in the core
Some BD articles worth noting
Interpreting
flow cytometry data: a guide for
the perplexed (Leonore A Herzenberg, James Tung, Wayne A Moore, Leonard A Herzenberg & David R Parks)